Large vision-language models (VLMs) have garnered increasing interest in autonomous driving areas, due to their advanced capabilities in complex reasoning tasks essential for highly autonomous vehicle behavior. Despite their potential, research in autonomous systems is hindered by the lack of datasets with annotated reasoning chains that explain the decision-making processes in driving. To bridge this gap, we present Reason2Drive, a benchmark dataset with over 600K video-text pairs, aimed at facilitating the study of interpretable reasoning in complex driving environments. We distinctly characterize the autonomous driving process as a sequential combination of perception, prediction, and reasoning steps, and the question-answer pairs are automatically collected from a diverse range of open-source outdoor driving datasets, including nuScenes, Waymo and ONCE. Moreover, we introduce a novel aggregated evaluation metric to assess chain-based reasoning performance in autonomous systems, addressing the reasoning ambiguities of existing metrics such as BLEU and CIDEr. Based on the proposed benchmark, we conduct experiments to assess various existing VLMs, revealing insights into their reasoning capabilities. Additionally, we develop an efficient approach to empower VLMs to leverage object-level perceptual elements in both feature extraction and prediction, further enhancing their reasoning accuracy. Extendable experiments demonstrate the supportive effect of Reason2Drive towards visual reasoning and downstream planning tasks. The code and dataset will be released.